What scales of antibody production does Biointron offer?

Biointron provides scalable antibody production services, ranging from milligram to gram levels, to accommodate various research, diagnostic, and therapeutic needs.

What expression systems are used for large-scale production?

Biointron utilizes mammalian cell systems, such as CHO-K1, for antibody expression, ensuring high yield and quality.

What is the typical timeline for large-scale antibody production?

The production process typically takes 2–4 weeks, depending on the project's complexity and specific requirements.

What quality control measures are implemented during production?

Biointron employs rigorous quality control protocols, including SDS-PAGE analysis (ensuring purity >95%), low endotoxin levels, and SEC-HPLC detection, to guarantee the highest standards.

Can Biointron produce antibodies in different formats?

Yes, Biointron can produce antibodies in various formats, including IgG, scFv, Fab, and bispecific antibodies, tailored to meet specific research or therapeutic needs.

What deliverables will I receive upon project completion?

Clients receive purified antibody samples, along with detailed reports on production processes, quality control results, and any relevant assay data.

How should I prepare and ship my samples to Biointron?

Biointron provides detailed instructions for sample preparation and shipping to ensure the integrity of your materials during transit.

How can I initiate a large-scale antibody production project with Biointron?

To start, contact Biointron through the website’s inquiry form or email. A consultation will be arranged to discuss project requirements and provide a tailored proposal.

二重特異性抗体生産

サービス概要

現在の競争激しいバイオ治療市場は主に抗体分子によって牽引されており、バイスペシフィック抗体(Bispecific Antibody)は次世代の抗体療法の重要な要素を表しています。血液循環中で最も一般的な抗体のタイプである免疫グロブリンG（IgG）は、通常はモノスペシフィックであり、IgG4を除いては単一の抗原のみを認識します。対照的に、バイスペシフィック抗体(Bispecific Antibody)は、同時に2つの異なるターゲットを認識して結合するように設計されています。

これは治療として有用です。例えば、T細胞などの免疫細胞を特異的に誘導して標的の腫瘍細胞を選択的に結合させ、排除させることができます。これにより、腫瘍細胞の受容体への効果的な結合が可能になり、同時に細胞毒性の免疫細胞を募集します。さらに、バイスペシフィック抗体(Bispecific Antibody)は、毒素や薬などの治療輸送物質を体内の特定の部位に送達するのに役立ちます。

Biointronは、1万以上のバイスペシフィック抗体(Bispecific Antibody)を発現しており、バイスペシフィック抗体(Bispecific Antibody)の製造における幅広い経験を持っています。私たちは、どんなクライアントの要望にも対応できる能力を誇りに思っており、バイスペシフィック抗体(Bispecific Antibody)の製造において幅広いフォーマットを提供しています。

ハイライト

カスタマイズサービス

どのような形式でも表現可能
お客様の研究プロジェクトに合わせたアプローチ

ラージキャパシティ

自動化された作業ステーション
精製とQCのための複数の方法

高い専門性

2～3週間（配列から精製されたBsAbsまで）
1万を超えるバイスペシフィック抗体が納品されています。

作業フローチャート

遺伝子合成

プラスミドの構築及び準備

トランジェント発現

アフィニティー精製

厳密な品質管理

速い配達

二重特異性抗体生産サービスの詳細

Our bispecific antibody production service follows a comprehensive workflow to ensure quality and efficiency at every step.

ステップ	詳細	作業時間	納品
遺伝子合成とクローニング	コドン最適化と遺伝子合成発現ベクターへのサブクローニングプラスミド増幅と調製	1週間	GOIを含む発現ベクター精製された抗体（エンドトキシンレベル<1EU/mg、SDS-PAGEとSEC-HPLCレポート） CoAレポート
トランジェント発現と精製	哺乳類細胞へのトランスフェクショントランジェント発現と精製 QC分析	2週間

遺伝子合成とクローニング
作業時間

1週間

詳細
- コドン最適化と遺伝子合成
- 発現ベクターへのサブクローニング
- プラスミド増幅と調製
納品
- GOIを含む発現ベクター
- 精製された抗体（エンドトキシンレベル<1EU/mg、SDS-PAGEとSEC-HPLCレポート）
- CoAレポート
Transient expression & purification
作業時間

2週間

詳細
- 哺乳類細胞へのトランスフェクション
- トランジェント発現と精製
- QC分析
納品
- GOIを含む発現ベクター
- 精製された抗体（エンドトキシンレベル<1EU/mg、SDS-PAGEとSEC-HPLCレポート）
- CoAレポート

事例研究

Case 1: 1-step purification of IgG-scFv

This case highlights the one-step purification of two IgG-scFv fusion constructs, with the scFv fused to either the N- or C-terminus of the IgG. Following 4-day expression, both constructs achieved high purity, demonstrating the efficiency of the one-step purification process.

Yield (mg/L)	SDS-PAGE	SEC (280 nm)
253.3	>95%	99.440%

Yield (mg/L)	SDS-PAGE	SEC (280 nm)
230.05	>95%	99.440%

Case 2:

This case involves the expression of an asymmetric multi-specific antibody with a molecular weight of 193 kDa. Following one-step Protein A purification, SDS-PAGE and analytical SEC-HPLC revealed the presence of half antibodies and hole-hole homodimers. Due to the close pI values, mixed-mode chromatography was employed for polishing, effectively removing most of the impurities.

Protein A purification

SEC-HPLC: 71.17%

Protein A purification

SEC-HPLC: 97.17%

Case 3:

This case involves the expression of a CrossMab^VH-VL. After one-step Protein A purification, the ratio of the target heterodimer was difficult to determine. Mass spectrometry analysis revealed impurities such as half antibodies and constructs containing identical light chains. To further purify the product, hydrophobic interaction chromatography (HIC) was applied, successfully removing most of the impurities.

Target heterodimer: 65%
By-products: HC1+LC1; HC1+HC2+2*LC1

2nd purification: HIC

Target heterodimer: 96%

FAQs About Bispecific Antibody Production

What is the typical timeline for producing bispecific antibodies?

Biointron delivers bispecific antibodies within 2–3 weeks from sequence to purified product, ensuring rapid project progression.
What materials are required to initiate a bispecific antibody production project?

Clients need to provide the sequences of the two monoclonal antibodies intended for bispecific antibody construction. If the sequences are unavailable, Biointron offers sequencing services to obtain them.
What formats of bispecific antibodies can Biointron produce?
Biointron can produce various bispecific antibody formats, including:
- Knobs-into-holes IgG
- Ig-scFv fusion
- Diabody-Fc fusion
- Dual-Variable-Domain-IgG This flexibility allows customization to meet specific research or therapeutic needs.
What expression systems are used for bispecific antibody production?

Biointron utilizes mammalian cell systems, such as CHO-K1, for antibody expression, ensuring high yield and quality.
What quality control measures are implemented during production?

Biointron employs rigorous quality control protocols, including SDS-PAGE analysis (ensuring purity >95%), low endotoxin levels, and SEC-HPLC detection, to guarantee the highest standards.
What deliverables will I receive upon project completion?

Clients receive purified bispecific antibody samples, along with detailed reports on production processes, quality control results, and any relevant assay data.
How should I prepare and ship my samples to Biointron?

Biointron provides detailed instructions for sample preparation and shipping to ensure the integrity of your materials during transit.
How can I initiate a bispecific antibody production project with Biointron?

To start, contact Biointron through the website’s inquiry form or email. A consultation will be arranged to discuss project requirements and provide a tailored proposal.

二重特異性抗体生産

サービス概要