サポート ブログ Immunoprecipitation

Immunoprecipitation

Biointron 2024-01-20 Read time: 2 mins
Immunoprecip.jpg
Image credit: DOI: 10.1007/s10540-005-2847-z

Immunoprecipitation (IP) is a small-scale affinity purification technique used to isolate and concentrate a particular antigen from a sample solution, which may contain numerous other proteins. This is achieved by using an antibody that recognizes the target protein and is immobilized to a solid support like magnetic particles or agarose resin. 

There are several different approaches, including the traditional (classical) method, oriented affinity method and direct affinity method. The traditional method of IP involves incubating the antibody with a sample, then binding it to Protein A or G agarose. However, this can lead to the target protein getting contaminated with the IP antibody, complicating subsequent analyses.

The oriented affinity technique uses Protein A or G beads as anchors, crosslinking the IP antibody to them. This prevents the antibody and target protein from being eluted together. In another approach, the direct affinity method attaches the IP antibody directly to a chemically activated base.

IP offers several benefits for scientists, such as detecting protein activation states, post-translational modifications, estimating molecular weights of proteins, and investigating protein-protein and protein-nucleic acid interactions.

At Biointron, we are dedicated to accelerating your antibody discovery, optimization, and production needs. Our team of experts can provide customized solutions that meet your specific research needs. Contact us to learn more about our services and how we can help accelerate your research and drug development projects. 


References:

  1. Kaboord, B., & Perr, M. (2008). Isolation of proteins and protein complexes by immunoprecipitation. Methods in molecular biology (Clifton, N.J.), 424, 349–364. https://doi.org/10.1007/978-1-60327-064-9_27

Subscribe to our ブログ

Recent ブログ

The therapeutic efficacy of antibodies is closely related to their ability to recognize and bind specific epitopes on target antigens. Epitopes, or antigenic determinants, are a group of amino acids or other chemical groups that are part of a molecule to which an antibody attaches itself. Epitope characterization can help reveal the mechanism of antibody binding and apply intellectual property (patent) protection for novel antibodies, in addition to designing antibodies with high specificity and minimal cross-reactivity.

Jul 12, 2024
ブログ

Understanding the differences between antibody specificity and selectivity is essential for designing and interpreting antibody-based assays in research for experimental accuracy and data interpretation. Antibody specificity refers to an antibody's ability to recognize and bind to a particular epitope—a unique part of an antigen that elicits an immune response.

Jul 10, 2024
ブログ

Antibody-based assays are essential tools in biomedical research, providing the means to detect, quantify, and visualize specific proteins or antigens within complex biological samples. These assays' efficacy hinges on the antibodies' precise properties. While affinity, avidity, specificity, and selectivity are fundamental to antibody performance, the ultimate impact of these properties is heavily influenced by the experimental context in which the antibody is employed.

Jul 08, 2024
ブログ

Biologics, particularly antibodies, have become indispensable in biomedical research and therapeutic development. Research-use-only (RUO) biologics play a pivotal role in preclinical studies, providing researchers with the necessary tools to explore antibody functions and therapeutic potential in vivo.

Jul 04, 2024
ブログ

お客様の利便性を向上させるためにクッキーを使用しています。詳しくは プライバシーポリシー をご覧ください。